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Dose–Response Curves for Cytotoxicity Testing on Human Skin Fibroblasts (HSFs). Dose–response relationships of rupatadine, DMSO (vehicle control), and doxorubicin (positive control) on HSFs cell viability. Cells were treated with increasing concentrations of test compounds for 2 h, and cell viability was assessed using the SRB colorimetric assay. IC 50 values were calculated using GraphPad Prism software. The dotted line indicates IC 50 of rupatadine (1150 µg/mL). Rupatadine demonstrated minimal cytotoxicity compared to the potent positive control doxorubicin, while DMSO showed no significant cytotoxic effects across all tested concentrations

Journal: AMB Express

Article Title: Repurposing rupatadine as topical treatment against methicillin-resistant Staphylococcus aureus

doi: 10.1186/s13568-025-01947-w

Figure Lengend Snippet: Dose–Response Curves for Cytotoxicity Testing on Human Skin Fibroblasts (HSFs). Dose–response relationships of rupatadine, DMSO (vehicle control), and doxorubicin (positive control) on HSFs cell viability. Cells were treated with increasing concentrations of test compounds for 2 h, and cell viability was assessed using the SRB colorimetric assay. IC 50 values were calculated using GraphPad Prism software. The dotted line indicates IC 50 of rupatadine (1150 µg/mL). Rupatadine demonstrated minimal cytotoxicity compared to the potent positive control doxorubicin, while DMSO showed no significant cytotoxic effects across all tested concentrations

Article Snippet: Rupatadine cytotoxicity was assessed using Sulforhodamine B (SRB) colorimetric assay on human skin fibroblasts (HSFs) (AcceGen Biotech Cat. # ABC-TC3451, USA; Obtained in September 2023).

Techniques: Control, Positive Control, Colorimetric Assay, Software